The parameters used were Rt range 0 23 min, mass range 100 1000 Da, mass tolerance 0.05 Da, internal standard detection parameters were deselected for peak retention time alignment, isotopic peaks were excluded for analysis, and noise elimination level was set at 6.00.
In addition to these settings, parameters such as mass detection range, sample resolution (sample acquisition rate in GS/s) and the laser firing rate; as well as the way the sample being prepared, i.e. homogeneity of crystallisation of the sample on the target plate, may also affect quality of the finished MS data.
The detection parameters were broadband detection, 512 K acquisition size, and start mass at m/z 200 leading to 0.5243 seconds transient duration allowing theoretical resolution of 190,000 at m/z 400.
The number of detected retention time/mass pairs depends on the peak detection parameters applied and varies with varying settings.
We break the problem down into three stages: mass detection, mass segmentation, and mass classification.
Important detection parameters, pH and detection temperature were optimized.
Recently, GBNRs have been used in mass detection applications.
Detection parameters are selected in the following way.
The mass detection range of CyTOF® covers close to 100 mass detection channels (CyTOF® instrument release 1), and offers an increase in the number of measurable parameters per cell, while obviating the need to perform compensation across channels.
Specificity for mass detection ranged from 80%to100%0%.
