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However, only a limited number of diameter combinations are provided since the mass data acquisition requires large amount of time.
Also we are developing a new mass data acquisition system (MDAS) that has simple architecture but has greater performance and flexibility.
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Mass data acquisitions were piloted by 4000 Series Explorer™ Software v3.0.
An autonomous metabolomic workflow combining mass spectrometry analysis with tandem mass spectrometry data acquisition was designed to allow for simultaneous data processing and metabolite characterization.
The tandem mass spectrometry data acquisition enabled automatic search and matching against the METLIN tandem mass spectrometry database, shortening the current workflow from days to hours.
Mass spectrometric data acquisition was performed in a data-dependent manner on a hybrid LTQ-Orbitrap mass spectrometer.
The general experimental workflow consisted of intact protein fractionation followed by mass spectrometric data acquisition by LC-MS/MS and data analysis, as described in Figure 1.
Undifferentiated and differentiated HL-60 cells (1×106) were washed with PBS, and frozen pellets were submitted for mass spectral data acquisition at the Medical University of South Carolina Lipidomics Facility.
Therefore, a different set of uninfected and infected lungs were separately homogenized, and an aliquot of the homogenate was used for the mass spectral data acquisition and another aliquot was used for the quantitation of GAPDH by Western blot using the LabWorks Image Acquisition and Analysis software from UVP BioImaging Systems, version 4.5.
RJC and ALB: Mass spectrometry data acquisition and analysis and manuscript preparation.
Each student was responsible for preparing their own digested protein sample and students contributed to creating mass spectrometry data acquisition methods.
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