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Mass calibration of the instrument is very simple and reproducible.
A mass calibration of the PIXE set-up has been done with MicroMatter thin film standards and other thick standards.
A lock mass calibration of sulfadimethoxin at a concentration of 0.05 mg/L in water:methanol (50 50, v/v) was introduced by HPLC pump (LC-10ATVP, Shimadzu, Japan) and split the 60 μL/min to lock spray interface.
External mass calibration of the hybrid mass spectrometer is performed using a standard calibration mixture of different peptides and matrix components.
TFA-Na cluster ions were used for mass calibration of the instrument prior to sample injection.
The external mass calibration of the orbitrap was performed every 3 days to ensure a working mass accuracy <5 ppm.
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This mass bias is consistent with previous WL mass calibrations of Planck clusters within the errors.
Quasi-internal mass calibration was used, by measurement of an infused calibrant prior to the compound of interest.
It also allows for the correction of mass calibration gaps [242] caused by regular switches between the analyte and a standard reference compound.
The background peaks from PBS/medium solution were used as internal standards to improve the accuracy of mass calibration.
To ensure accuracy and reproducibility, all analyses were carried out using leucine enkephalin for lock mass calibration at a concentration of 0.4 ng/L, in 50/50 ACN/H2O with 0.1% v/v formic acid.
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