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Subsequently DNA methylation levels at RAMP1 promoter region were measured using Sequenom Mass ARRAY systems.
Followed treatment with shrimp alkaline phosphatase, T7 polymerase/ribonuclease A and desalting, PCR production was conducted with Mass ARRAY EpiTYPER system (Sequenom, CA, USA) according to manufacturer's instructions.
This gyroscope consists of a two-port SAW resonator, with a vibrating mass array within the cavity, and two SAW oscillators, one of which is used as a sensor and the other as a reference.
The EpiTYPER Sequenom Mass Array [53] service was provided by Genetic Services, Sequenom, Inc.
The SNP genotyping method used the Mass Array system to design multiplex reactions for PCR and iPlex primer extension (Sequenom) and the MALDI-TOF based Mass Array genotype platform (Sequenom).
In addition, despite their much lower WAT mass, array results indicate the presence of chronic inflammation in adipose tissue in the absence of obesity.
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The correlation between SNP-specific microarray and mass ARRAY-based techniques is shown with high R2 value (0.84) at p-value < 0.001 in Additional file 6. Cotton fibers were mechanically separated from frozen ovular tissue using a modified liquid nitrogen and glass bead shearing procedure [ 47].
One of the most widely used omics technology platforms is mass spectrometry based detection of somatic gene mutations using the Sequenome DNA mass-array technology.
Genotyping of Stage II samples was done using Sequenom Mass-ARRAY iPlex platform.
For Stage II, 1,153 breast cancer cases and 1,215 controls were genotyped using Sequenom Mass-ARRAY iPlex technology (Gabriel et al. 2009).
Genotyping was performed using the Sequenom iPLEX Mass-Array assay system in a 384-well format according to the manufacturer's instructions.
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