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A reason might be that the available reference ligands are very small (average molecular mass approx. 194 g/mol) and thus undergo a limited number of interactions, including two hydrogen bonds (Fig. 4a).
Additionally, a band of mass approx. 5 kDa lower than the uppermost band in the wild-type lane was present, representing EndoH-resistant protein.
CVF is a protein with high molecular mass (approx. 150 kDa) and it dissociates into α, β and γ chains under reducing condition [ 64], consistent with our findings where the gel sections 3, 5, 6 and 7 (Fig. 3) corresponded to the reported sizes of α (72kDa), β (45kDa) and γ chain (32kDa).
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In this case, the kinematics of the snake robot corresponds to a planar snake robot with links of length (2l = 0.18) m and mass (m approx 0.8) kg.
MyD88 exists as a homodimer with a molecular mass of approx. 62 66 kDa but could also be detected as a monomer with a molecular mass of 31 33 kDa in SDS-PAGE [37].
In contrast, addition of SpeB to the reaction led to a complete conversion of CXCL9 into a fragment with an apparent mass of approx. 10 kDa (Fig. 3A, lane B).
The main activity band was observed from a protein with an apparent mass of approx 95 kD (Fig. 6, lane 2).
The Pgm protein eluted at a volume between 73.8 and 74.3 ml, corresponding to a molecular mass of approx. 122 kDa.
TRAP (trp RNA-binding attenuation protein) is a highly thermostable, ring-shaped homo-11mer with a molecular mass of approx. 91 kDa [ 12].
By MALDI TOF-MS peptide fingerprinting, the band with a MALDI TOF-MSss of apeptide60 kDa could be assigned to cg2800, which was annotated as pgm.
The 200 kDa complex is probably a dimer of SPP-dimers, since we detect dimers after 2D-BN-SDS/PAGE and since one SPP dimer has a molecular mass of approx. 90 kDa [ 16].
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