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This in combination with data with good mass accuracy (in raw data or following post-acquisition mass alignment) results in a greatly reduced number of putative metabolite identifications within a specified mass tolerance (typically 3 p.p.m. for the ThermoFisher Scientific LTQ-Orbitrap acquired data).
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We compare the reconstructions from alignment using cross-correlation and our center-of-mass technique, while also demonstrating the advantage of using many slices for the center-of-mass alignment, as opposed to just one center-of-mass calculation.
The plot in Figure 6g shows the only consideration for the originally proposed center-of-mass alignment, as the center of mass in the projections along the y-axis is shifted to the tilt axis.
In target shooting there are two approaches to sight alignment: Center of Mass (aim at the center of the bullseye) or 6 o'clock hold (aim just underneath of the bullseye).
Sight Alignment.
Each dataset was processed using MetAlign (http://www.metalign.nl) for baseline correction, noise estimation, and ion-wise mass spectral alignment of the corresponding chromatograms.
In P. aeruginosa twitching motility and M. xanthus social motility, cells that form a motile mass undergo alignments along the long axis of the cells upon coming into contact with each other to organize into raft-like clusters [43], [44].
The latter case is particularly true when binding sites are highly or perfectly conserved in which case the bulk of the probability mass in the alignment distribution rests on a single alignment.
Hierarchical control forces, exerted via coil-magnet actuators placed at three different stages of the SA, damp the fundamental mechanical modes of the system (all below 2 Hz) and maintain the relative orientation and position of the test mass for interferometer alignment and locking.
The chromosomes looked like rounded mass without proper alignment at the metaphase plate (Additional file 1, Figures S1 and S4).
Furthermore, of the mass of structural alignment methods [ 6- 34], hardly any explicitly try to estimate log-odds probabilities as a similarity measure [ 35].
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Justyna Jupowicz-Kozak
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