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What is the mass accuracy of the measurement (see below)?
In the early 1960's, mass accuracy of MS instruments was relatively low.
In practice, this situation will show up extremely rarely for mass accuracy of 10 ppm or better.
On the other hand, we demand that the mass accuracy of the measurement is high, say, 20 ppm or better.
In this evaluation, BRAIN outperformed all other software tools but Emass in mass accuracy of the isotope peaks.
This refers to the mass accuracy that we can expect in everyday use of the instrument, not to the "anecdotal mass accuracy" of a single measurement [53].
After importing the tandem mass spectra of a compound, all molecular formulas within the mass accuracy of the parent peak are generated (3).
A mass accuracy of 0.3 Da and a single missed cleavage were allowed for each matching peptide.
The mass accuracy of the instrument for intact proteins was set to be 100 ppm in the analysis.
The database search results were assembled and filtered using the DTASelect program with a spectra level false discovery rate of less than 0.5%, mass accuracy of 5 ppm.
A near point external calibration was applied for the MS/MS spectra and gave a typical mass accuracy of ∼50 ppm or less.
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