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Exact(17)
Mass accuracy in MS/MS mode was better than ±100 mDa.
Peak shape and mass accuracy in LC/MS measurements obtained employing the novel method appear to be similar to those observed in traditional magnitude-mode FT spectra.
The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification.
Δmmu: absolute mass accuracy in milimass units.
Increased sensitivity and mass accuracy in conjunction with comprehensive database annotations allows the high-throughput identification of proteins.
We have deliberately ignored that the mass difference between two measured peaks in ({mathcal{D}}) may be smaller than twice the chosen mass accuracy; in this case, two peaks would be assigned the same molecular formula in the fragmentation graph and, possibly, also the maximum colorful subtree, violating our condition that all nodes have to be different molecular formulas.
Similar(43)
With the present technology the technique does afford a high sample amount, protein fractions with a largely reduced complexity of composition, and high mass accuracies in the low ppm range.
The most widespread tandem mass analyzer providing high mass accuracy used in the metabolite identification is the hybrid double-quadrupole TOF instrument [ 19, 64, 77, 101, 112].
Label-free protein expression is a peptide-based proteomic technique that capitalizes on the highly reproducible chromatography and accurate mass accuracy available in current LC/MS systems.
First, UVPD mass spectra derived from monoubiquitin and the unmodified Sic60-DHFR construct were compared to confirm that there was no significant fragment ion overlap that would prevent assignment of diagnostic fragment ions and that could not be overcome by the high mass accuracy used in this study (see Figure 6).
Continual advances in mass accuracy and sensitivity in mass spectrometry greatly facilitates protein identification.
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