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Both the seed and waypoint masks were drawn on four consecutive axial slices in each subject's original T1-weighted map.
The waypoint masks and seed masks were drawn on the color map of the diffusion MRI data transformed to each subject's T1 space using a rigid-body transformation.
Separate masks were drawn for the rostral and dorsal portions of the anterior cingulate, consistent with prior findings suggesting that these sub-regions are differently affected in MDD [19], [20].
The segmentation masks were drawn in the entire image frame and not just in the FoI.
To calculate lifetime values from different regions within the same image, manually delineated region of interest masks were drawn from the white light reflectance images and applied to the processing of the fluorescence decays.
Using Novel multimask software, masks were drawn for each ROI and, in the same foot, for each of 10 anatomical foot regions: medial and lateral heel, medial and lateral midfoot, metatarsal 1, metatarsals 2/3, metatarsals 4/5, hallux, toes 2/3, and toes 4/5.
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Gorilla In A Pink Mask was drawn on the side of a former social club in Eastville.
For the PLIC ROI, the mask was drawn at z = 7.5 mm, where the PLIC could be clearly seen on both FA maps and eigenvector color maps.
For the PrG ROI, a mask was drawn on four consecutive axial slices approximately spanning the "hand knob" (z = 35.4 mm) [45].
A mask was drawn around the protein to exclude membrane and peptidoglycan from this estimate.
A mask was drawn around the neuromast based on the brn3c-gfp labeling, and the average intensity was calculated.
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