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We also masked the repeat sequences using Exonhunter (http://lifecenter.sgst.cn/schistosoma/en/schistosomaCnIndexPage.do) and RepeatMasker programs [26] (http://www.repeatmasker.org), and ones mapped to protein-coding exons.
RepeatMasker masked the repeat elements in the newly assembled genome using a custom library.
For zebrafish, C. elegans, D. melanogaster, and D. pseudoobscura, we masked the repeat regions to avoid the misassignment of duplogs due to repeat sequences.
We only see slight differences between Figure 3C and Figure 3A, but the change is far from that which occurred when masked the repeat sequences.
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RepeatScout uses five steps to investigate and mask the repeat elements within the genome.
The RepBase library of RepeatMasker and the non-redundant library of ab-initio classified repeats were then used to mask the repeat elements in the assembled genome.
One may argue that the RepeatMasker program might not mask the repeat sequences completely because of the limited amount of repeat elements available in the public database; however, this does not appear to have affected the BLAST results significantly.
The following steps consisted of masking the repeats, vector and organelle sequences.
To test this hypothesis, we retrieved the complete chromosomal sequences of the green anole from Ensembl, masking or not masking the repeated elements.
RepeatMasker (A.F.A. Smit, R. Hubley, and P. Green; RepeatMasker at http://repeatmasker.org, last accessed July 22 , 2014 was used to mask the repeats predicted from the above steps.
Homology among BAC clones from the same X/Y probe gene pair was assessed by aligning the BAC sequences with MAUVE 2.3.1 [ 32] after masking the repeats using RepeatMasker v3.3.0 (http://www.repeatmasker.org/) with the Silene DNA repeat database mentioned above.
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