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Human osteoprogenitors from bone marrow were selected using the antibody STRO-1 utilising a magnetically activated cell separation system.
Regions of interest (ROIs) corresponding to MCs were selected manually on the unenhanced image; control ROIs in the "healthy" bone marrow were selected.
The time points of days 1, 3, 5, 7, 10, 14, 28, and 56 days post-ablation of marrow were selected based on knowledge of the complete timeline of all general bone healing processes involving phases of inflammation, repair and new bone formation, and remodeling.
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CD138-positive bone marrow plasma cells were selected to a purity >95% using magnetic assisted cell sorting (Miltenyi Biotech, Bisley, UK).
The hematopoietic tissues (fetal liver, bone marrow, spleen, and thymus) were selected to evaluate by qRT-PCR whether potential compensation for the loss of Stfa2l1 and Csta in mutant animals could occur through increased mRNA levels of Stfa2 and Stfa3 (expressed at significant levels in these organs) as well as Stfa1 (highly expressed in wild-type fetal liver cells) (Figures 2B, S1, and 4A D).
Eighty-five Iranian patients (mean age of 32 ± 2.7 years, range of 14 49 years) with HD from bone marrow transplantation department of Taleghani Hospital were selected.
Primary myeloma plasma cells were selected from bone marrow of patients with MM using CD138 immunomagnetic bead separation [12].
MSCs were selected from the marrow aspirate on the basis of their ability to adhere to tissue culture plastic.
Four bone marrow samples containing >70% leukemic blasts were selected for each leukemia subtype analyzed (M3 and M5, according to the FAB classification).
Thirty-two consecutive patients positive for ITC in either blood or bone marrow and diagnosed with a potentially resectable tumour were selected for this study.
In 14 patients, bone marrow was harvested from iliac crest and BM-MNCs were selected by Ficoll gradient.
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