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Mice were injected with 2 mg BrdU i.p. 16 h later, and spleens and bone marrow were removed and made into single-cell suspensions.
The mice were monitored for changes in behavior and body weight for 1 month, after which they were killed; their heart, brain, liver, kidneys, bladder, and bone marrow were removed, fixed in buffered formalin, sectioned, stained with hematoxylin and eosin (H&E), and analyzed histologically.
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Bone marrow was removed with saline lavage.
BMX was performed on both tibias of each rat according to the method described by Suva et al. After the proximal region of each tibia had been exposed, a 1.5 to 2.0-mm hole was drilled in the cortex below the proximal metaphysis, and the marrow was removed via several rounds of suction and flushing with sterile saline.
To isolate RNA from whole bones or whole OS, bone marrow was removed and bones were mechanically crushed with a mortar and pestle and RNA extracted using TRIzol reagent (Invitrogen, Carlsbad, CA) according to standard protocol.
Briefly, marrow was removed from the long bones and cells plated out in cell isolation media (CIM) RPMI-16400 (Lonza, UK) with 9% FBS, 9% horse serum (both Gibco, UK)) at 37°C, 5% CO2.
Bone marrow was removed from trabecular bone by the routine technique used in our bone bank.
Bone marrow was removed from the femurs with 5 mL of hypotonic solution (0.075 M potassium chloride - Labsinth, São Paulo, Brazil).
Marrow was removed from the patients immediately before melphalan administration and returned i.v. 8 h later.
Using a syringe, bone marrow was removed and resuspended in 10 ml serum-free L-DMEM medium containing 100 IU/ml penicillin and 100 μg/ml streptomycin.
Next, bone marrow was removed from all the FDs, which then were first soaked with ether (to remove fat content) and later in H2O2 (to remove remaining blood deposits).
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