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Ectopic ossicles containing cortical bone, trabecular bone, and a hematopoietic marrow were generated from implanted bone marrow stromal cells (BMSC).
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Primary MSCs derived from human fetal bone marrow were transfected with a retroviral vector encoding v-myc oncogene (Figure 1A), and clones of immortalized human bone marrow MSCs were generated (see Methods).
Bone marrow chimeras were generated using the Ly5.1/5.2 reconstitution system, as previously described [6].
Bone marrow chimeras were generated to test the possibility that lamin A/C expression is required in developing lymphocytes for normal T and B cell maturation.
Bone marrow DC were generated from DA and BN rats, RBC lysed and cultured in RPMI media with 5% FCS, (rr) GM-CSF (50 ng/ml), and (rr) IL-4 (20 ng/ml) for 5 days.
PLPtg/PD-1-deficient double mutants and the corresponding bone marrow chimeras were generated and analysed using immunohistochemistry, light- and electron microscopy, with particular emphasis on immune-cell number and neural damage.
The DC from mouse bone marrow (mDC) were generated as described previously.
Bone marrow chimeras were generated by lethal irradiation of recipient mice with 8 Gy for 10 min, followed by retro-orbital injection with 8 × 10 nucleated donor bone marrow cells 6 h later.
Mixed bone marrow chimeras were generated by intravenously transferring 3 × 10 to 5 × 10 cells from the following mixes into lethally irradiated (2 × 450 rads, 3 hr apart) CD45.1+ congenic mice: blt/ blt CD45.2+: WT CD45.1+CD45.2+; blt/+ CD45.2+: WT CD45.1+CD45.2+, at a 50 50 ratio.
To determine if the expression of CD38 on p110δD910A Treg is governed by signaling within the T cells themselves or by an extrinsic factor, competitive bone-marrow chimeras were generated.
Bone marrow chimeric mice were generated by transferring isolated bone marrow from PHIL (PHIL->WT) or WT (WT->WT) donor mice into the retro-orbital plexus of sublethally irradiated WT recipients, as described previously.
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