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Activities in bone marrow were estimated from thoracic and lumbar vertebrae.
The residence time (h) of bone and the bone marrow were estimated using the reported method [38].
Animals were sacrificed and parasites in liver, spleen and bone marrow were estimated 4-wk post-treatment by microscopic examination of stamp smears and limiting dilution assay.
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The conjugate view method was used to quantify the activity, the accumulated activity was calculated and the absorbed dose to the bone marrow was estimated according to the MIRD scheme.
A value of 4 Gy was chosen as the MTDBM in the present study, and the absorbed dose to the bone marrow was estimated using bone marrow uptake data from a previous biodistribution study of 177Lu-BR96 in the same animal model [27].
The mean absorbed dose to the bone marrow was estimated to 0.20 Gy (0.11 0.37 Gy) per 7.4 GBq of 177Lu-DOTATATE, and the mean dose per fraction correlated with a decrease in Hb (p = 0.01), WBC (p < 0.01) and PLT (p < 0.01) counts.
Residence time for the red marrow was estimated by assuming a red marrow radioactivity concentration of 30% of the whole blood activity concentration [ 19].
By quantitatively analysing the In-IMP288 scintigraphic images and pharmacokinetics, the radiation dose to the kidney and bone marrow was estimated.
Given that the ratio of MSCs to other cells within the bone marrow is estimated at one in 10,000 cells, expansion is beneficial and must be optimized to ensure that an adequate yield of pure MSCs is available for implantation [ 105].
Volume fractions of bone, fibrous tissue, ABM/P-15 remnants, and marrow cavity were estimated by using point-counting technique [ 25].
In univariate analysis, differences in ratios between patients with and without bone marrow involvement were estimated with Student's t-test (for sequential samples in the same patients the paired t-test was used).
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