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Main Outcome Measures: Average T2 values from T2-weighted MR images of the extensor carpi radialis brevis (ECRB) and the extensor carpi radialis longus (ECRL), flexor digitorum profundus (FDP), and radius marrow were determined across 7 sections and 4 exercise bouts and a preexercise condition.
The absolute cell numbers of recipient-origin stem cells (CD45.2+ KLS cells) and donor derived stem cells (CD45.1+ KLS cells) in the spleen and in the marrow were determined, as described above.
Plasma cell numbers in the spleen and bone marrow were determined 12 hours and 3, 7 and 15 days after the last bortezomib treatment.
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Dose to red marrow was determined from blood concentrations using a baseline value of 0.1 [21].
After bone-marrow transplantation from a female donor, the cellular chimerism in the bone marrow was determined as 60% donor versus 40% recipient cells using a centromeric probe for the X chromosome.
Calculation of radioactivity in the bone marrow was determined assuming each femur represents 6% of total bone marrow (19).
The copy number of BCR ABL transcripts in bone marrow was determined at the central laboratory using the RQ-PCR as described previously.
It has been shown that the number of plasma cells in human and mouse bone marrow is determined by the number of chemokine (C-X-C motif) ligand 12 (CXCL12 -expressing stromal CXCL12 -expressingize stromall nicellsfor individual LLPCs [ 24].
The numbers of CFU-Fs in murine bone marrow isolates were determined in cocultures with irradiated guinea pig marrow cells, as reported.
Epo levels (R&D Systems, Minneapolis, MN) of the animal serum, Alkaline phosphatase (ALP) activity levels (Sigma-Aldrich, St . Louis MO) of the cell lysates, and CXCL12 levels (R&D Systems) of the bone marrow supernatants were determined by double-antibody sandwich method assembled with commercially available components, according to the directions of the manufacturer.
The bone marrow differentials were determined by flow cytometry as described by Saad et al [ 23] and the total nucleated cell count, the myeloid: erythroid ratio and the proportion of lymphoid, myeloid, erythroid and nucleated cells were determined.
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