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CD34+ cells in bone marrow were assessed by flow cytometry.
On day 31 inflammatory responses in bronchoalveolar lavage, airway sections and lung tissue, and eosinophil numbers in the bone marrow were assessed as previously described [28] [30].
To determine the point of the inflammatory pathways where eosinophil influx in the lung was disrupted in STAT6-deficient mice, eosinophil levels in the blood and bone marrow were assessed.
Monoclonality and percentages of κ/ λ neoplastic cells in the bone marrow were assessed by in situ hybridisation.
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The multilineage engraftment in the bone marrow was assessed by immunostaining the BM cells with a panel of antibodies against human lineage markers comprising CD33 (myeloid), CD41a (megakaryocyte), and CD19 (B lymphoid).
To assess the capacity of CD34+ cell3+ cell number to predict SCID repopulating activity before and after culture, 400, 1000 and 5000 uncultured CD34+ cells+ cells and the total cultured cells containing 400, 1000 and 5000 CD34+ CD133+ cells were injected into neonate NSG mice and the chimerism in the bone marrow was assessed 12 weeks after injection.
Bone marrow was assessed by morphology on day 16.
Micrometastasis of human cancer cells to mouse kidney, liver, lung, brain and femurs (bone marrow) was assessed at 3 months post-injection using PCR to detect human chromosome 17 in the mouse tissues.
SUVs of the pathological lesions and of the liver, spleen, lung, blood, skeletal muscles, bone marrow, gluteal fat, abdomen and heart were assessed.
In addition, radioadaptation and bone marrow transplantation were assessed as interventions to mitigate the skeletal complications of irradiation.
Patients who developed grade 4 bone marrow suppression were assessed every 1 2 days.
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