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Exact(6)
Then, both ends were cut with scissors and the marrow was washed with PBS through a syringe.
The supernatant was discarded and the marrow was washed two more times in D10, resuspended in conditioned media (DMEM with 20% (v/v) FBS, L-glutamine, penicillin/streptomycin and 10% (v/v) supernatant from the L929 hybridoma cell line [42]), placed in a 75 cm2 tissue culture flasks and incubated at 37°C with 5% CO2.
The bone marrow was washed out with phosphate-buffered saline under sterile conditions.
The resulting diluted bone marrow was washed with L15/10% FCS and pelleted.
Bone marrow was washed out from legs tubular cylindrical bones with 75 mM KCl. Suspension was incubated 15 min in 37°C and centrifuged 5 min 1000 rpm.
Near the end of the study, all mice were dissected and bone marrow was washed with 100 μL phosphate buffer solution (PBS) via the flashing method on the femur of sacrificed mice [ 27].
Similar(54)
Blood and bone marrow were washed once with sterile phosphate buffered saline (PBS) and subjected to red blood cell (RBC) lysis, according to manufacturer's instructions (RBC lysis buffer, Biolegend).
Each marrow sample was washed with PBS.
The marrow sample was washed with Dulbecco's phosphate-buffered saline (DPBS), and disaggregated by gently passing the sample through a 21-gauge catheter and syringe to obtain a single-cell suspension.
Animals were killed 72 hours after shock; the marrow cavity of the femur was washed with McCoy's Medium (2 ml), and total and differential leukocyte counts were performed in the perfusate.
The femoral bone cavity was washed with low-sugar DMEM medium to harvest bone marrow cells.
More suggestions(16)
marrow was discarded
marrow was flushed
marrow was collected
marrow was aspirated
marrow was given
marrow was calculated
marrow was diagnosed
marrow was injected
marrow was observed
marrow was examined
marrow was invaded
marrow was mixed
marrow was extruded
marrow was extracted
marrow was harvested
marrow was cooked
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