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In the reciprocal experiment, wild type or PI3Kγ−/− bone marrow was transferred into PI3Kγ−/− recipients; in these bone marrow chimeras we again observed comparable infarction sizes (Figure 3B, C) and Troponin T release (Figure 3D).
Propylene oxide was allowed to evaporate over 4 h before the marrow was transferred into fresh Epon and rotated overnight.
The volume of aspirated bone marrow was transferred immediately to vacutainer tubes containing 10.8 mg K2EDTA as an anticoagulant (Becton Dickinson, Franklin Lakes, NJ, USA).
By contrast, when TNFΔARE bone marrow was transferred to TNFRI-/-recipients, no signs of joint inflammation were found, although gut inflammation was present.
This fact dictated the time course for analysing the current experiments, since we were restricted by the survival time of mice where MyD88 KO bone marrow was transferred into irradiated MyD88 KO recipients (KO→KO).
When wild-type marrow was transferred to Sucnr1−/− mice, the number of subretinal microglia was ~10-fold higher (P= 0.0217) while transfer of Sucnr1−/− to wild-type hosts did not increase microglial levels above wild-type controls.
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In May 2000, a patient colonized with VRE in the bone marrow transplant unit was transferred to the PICU.
The presence of Ab titers to TT, DT, and MV in the serum of recipients and the production of in vitro anti-TT by T and B cells from recipients show that Ag-specific memory was transferred via the marrow inoculum.
On day 81, he was transferred to undergo bone marrow transplantation.
On day 62, a CT scan showed no abscess around the resection stump, and on day 81, he was transferred to undergo bone marrow transplantation.
Bone marrow samples were obtained from voluntary donors at the Bone Marrow Transplant Unit, Hematology Service, Hospital Universitário Clementino Fraga Filho HUCFFF) at UFRJ by washing with phosphate buffered saline (PBS) the bone marrow collection kits after bone marrow aspirates were transferred to infusion bags.
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