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The femoral bone marrow was perfused as previously described.
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The jejunal segment was perfused with buffer containing [3H] propranolol.
Phosphate buffer (10 mM, pH 7.4) was perfused at a flow rate of 2.0 μL/min.
The liver was perfused with PBS, weighed, and the tissue used for RNA isolation.
Each patch was perfused with 1 μM ChTX as indicated by the horizontal bars.
After explant, saline was perfused through all grafts and the hydrodynamic resistance was quantified.
The arterial tree was perfused with PBS.
Each concentration was perfused for 5 min.
The rat was perfused 1 week after infusion of tracer.
For live cell imaging, the endothelial monolayer was placed in the flow chamber and highly purified bone marrow derived PMNs were perfused over the pMBMECs.
At the indicated time points post-infection (i.e. 18 or 24 h), MyD88 bone marrow chimaera mice were perfused transcardially to eliminate leucocytes from the vasculature, whereupon brains were removed and immediately flash-frozen on dry ice.
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