An 18-gauge needle was inserted into the proximal end of each bone, and the marrow was flushed into sterile cryovials and stored on ice.
Whole bone marrow was flushed from the femurs and tibias.
Briefly, the femurs were collected aseptically from mice and the marrow was flushed out.
Bone marrow was flushed from the femurs, tibia, and humeri with Hank's buffer salt solution (Invitrogen).
In brief, bone marrow was flushed from the femurs and tibias of mice.
Bone marrow was flushed from mice femurs and tibias aged eight to twelve weeks.
Briefly, femurs were cut off at the ends and the marrow was flushed out.
Bone marrow was flushed from femurs using a syringe with a fine gauge needle and 3 mL of PBS.
4-months post transplantation bone marrow was flushed from a mouse transplanted with 1×108 human TNC cells.
Briefly, rats were sacrificed, and bone marrow was flushed from femurs and tibias using 10 ml of DMEM in a 10 ml syringe with a 26-gauge needle.
Briefly, bone marrow was flushed from femurs and tibias with Dulbecco's modified Eagle's medium (DMEM)(Gibco, Invitrogen, USA) and centrifuged at 600 g for 10 minutes.
