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Henry's bag of marrow was collected from an anonymous donor somewhere in the United States on the morning of July 6, 2000, and was flown to the Fairview-University Medinal CenteRoomrriving in Room 5 of the bone-marrow transplant floor around dinnertime.
For analysis of HSCs, whole bone marrow was collected from the femurs and tibias of mice.
Bone marrow was collected from an eighteen-year old healthy female donor.
Bone marrow was collected from the femurs of either wild-type female C57BL/6J or KitW-sh/KitW-sh mice.
Briefly, bone marrow was collected from tibias and femurs of 6- to 7-week-old female NOD/ShiLTJ mice.
Briefly, bone marrow was collected by flushing femur and tibia with medium and single cell suspensions prepared by repetitively pipetting through 18-gauge needles.
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At the end of the experiment, liver, testis, serum, and bone marrow were collected.
Blood, organs, and femur-containing femur marrow were collected and counted as described previously.
Blood and bone marrow cells (marrow) were collected and stained with anti-Ly-6G-FITC, and anti-CD177-PE antibodies, followed by flow cytometry, n = 3 Open image in new window Figure 3 Normal development of myeloid cell lineages inCD177 −/− mice.
Lung samples, spleens, and bone marrow were collected at day 4 post challenge.
At various times after immunization, mice were anesthetized with isoflurane (Abbott Laboratories, North Chicago, IL) and blood, draining popliteal LNs, spleens and bone marrow were collected.
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