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Genomic DNA was extracted from 2 ml frozen whole blood or bone marrow using the RelaxGene Blood DNA system according to the manufacturer's protocol (Tiangen, China).
Additionally, RD114 provirus was not detected in genomic DNA extracted from peripheral blood, lymph nodes, spleen and sternal bone marrow, using the one-step PCR assays.
MSCs or skeletal stem cells were enriched from human bone marrow using the STRO1 antibody and magnetic activated cell sorting (MACS) as previously described [2].
In other words, a 42-fold increased yield of early-stage BM-MSCs could be produced from bone marrow using the gelatin matrix.
Mesenchymal stem cells were isolated from human donor red cell-enriched rib bone marrow using the RosetteSep human MSC enrichment cocktail and cultured as defined by Gazi et al (2007).
Bone marrow was obtained at the end of the experiments from a number of randomly selected mice and used to determine the in vitro sensitivity of the grafted bone marrow using the in vitro bone marrow toxicity test.
Similar(54)
This needle was removed and 0.1 ml of saline was injected into the cavity to flush out the marrow using a 30G needle.
This suggests a role for RT-PCR in the detection of colon carcinoma metastasis in blood and bone marrow, using CK 20 as the target gene.
In 38/100 patients (38%), tumour cells were detected in the marrow using an immunocytochemical technique with a cocktail of two monoclonal antibodies: anti-EMA and anti-cytokeratin.
Another aspect is the possible difference in the kinetics of the radiopharmaceutical in the low uptake compartment compared to the bone marrow, which may lead to an overestimation or underestimation of the absorbed dose to the bone marrow using a fixed ratio.
Mast cells were immunomagnetically isolated from the peritoneal lavage and from the bone marrow using mAb AA4 coupled to tosylactivated Dynabeads M-450 as previously described [25], [26].
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