Sentence examples for marrow specimens using from inspiring English sources

In additional to conventional cytogenetic analysis, fluorescent in situ hybridization (FISH) was applied in appropriate bone marrow specimens using 13q14.3, 13q24, and 17p13.1 (p53) deletion probes and 14q32 (IGH) break apart probe (Vysis, Des Plaines, IL, USA) according to the manufacturer's instructions.

Two types of genetic information were assessed in this case-comparison study: a) We collected and assessed all available information about the gene or chromosomal abnormalities characteristic of a child's leukemia cells that were ascertained at case presentation from bone marrow specimens and used for diagnosis and classification of leukemia.

Specimens used in this study.

Much of our knowledge of the resistance phenotypes in ALL has been derived using isolated bone marrow specimens studied in short-term culture using the 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) assay.

To obtain primary myeloma cells, CD138+ cells were isolated from bone marrow specimens obtained through the Norwegian Myeloma Biobank using RoboSep automated cell separator and the Human CD138 Positive Selection Kit (StemCell Technologies, Grenoble, France).

In a subset of 31 MM bone marrow specimens, analyzed for PTEN gene promoter hypermethylation, total RNA was isolated using Trizol reagent.

DNA was extracted from serum (n=4), blood (n=25), blood culture (n=9), and bone marrow (n=2) specimens by using the chaotropic properties of guanidine isothiocyanate, followed by DNA extraction and purification by alcohol precipitation with the IsoQuick Nucleic Acid Extraction Kit (ORCA Research, Bothell, WA).

Bone marrow specimens (3 5 ml) from 35 healthy bone marrow donors were used as negative controls to test the specificity of the RT-PCR assays.

Gradient density centrifugation using ficoll hypaque and purification by CD138-immunomagnetic beads were used to obtain purified malignant plasma cells from MM bone marrow specimens after written informed consent given at the University Hospital of Nantes.

Peripheral blood or bone marrow specimens were collected, processed and archived as part of standard clinical care using site-specific procedures.

Detection of F. tularensis in hare specimens, including bone marrow specimens, and lack of F. tularensis detection in samples of the water system used to rinse hares suggest infection of the hares.