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Testing by qPCR of 18 bone marrow specimens from paediatric acute lymphoblastic leukaemia (ALL) patients at diagnosis revealed nine to be GG, six to be GD and three to be DD for exon 2 of p14ARF/p16INK4A, concordant with Southern blotting analysis.
Cytokeratin-positive DTC were detected at diagnosis in bone marrow specimens from all patients.
Peripheral blood or bone marrow specimens from leukemia patients were collected, processed and archived at two independent sites.
Non-metastatic bone marrow specimens from three out of four other patients were strongly positive for Fc, mostly Feb.
In addition, a subset of bone marrow specimens from patients with AL amyloidosis seen at the Amyloid Treatment and Research Program at Boston University Medical Center were evaluated.
In addition, expression of BHRF1-1 and miR-155 by miRNA-ISH was found diffusely positive in bone marrow specimens from patients.
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The selected control was a bone marrow specimen from a healthy donor and was considered as 100% expression.
The selected control was the bone marrow specimen from a healthy donor.
The bone marrow specimen from the GAGE-2 seropositive patient was found negative for GAGE expression by IHC.
In a bone marrow specimen from a 2-year-old girl, we observed Fc, most of them Feb, distributed as foci and as single cells.
Of note, the bone marrow specimen from the GAGE-2 seropositive patient was found to be negative for GAGE by IHC.
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