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However, staining marrow sections from the IL7promECFP reporter mouse with antibodies against markers reported for osteoblasts (osteocalcin, osteopontin or SDF-1) failed to give specific staining as determined by an experienced immunohistochemistry service at NCI.
SLAM staining was performed in bone marrow sections from neonate mice.
We have stained osteocytes with phalloidin in bone marrow sections from Wnt1-Cre2 R26-tomato Wnt1-Cre2 R26-tomato Wnt1-Cre2 R26-tomato
(D ) Representative bone marrow sections from wt and Erbb3 -null E17.5/18.5 mice immunostained with anti-CD90 (red).
(C – C ′′) Fluorescent signals of GFP, Tomato, and DAPI in bone marrow sections from 1-week old Wnt1-Cre2 R26-Tomato Nes-Gfp Wnt1-Cre2 R26-Tomato Nes-Gfp Wnt1-Cre2 R26-Tomato Nes-Gfp Wnt1-Cre2 R26-Tomato Nes-Gfp
However, these antibodies could not detect metastatic tumour cells in the bone marrow sections from patients in whom CE did not reveal any tumour cells.
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(G ) Femoral bone marrow section from newborn Nes-Gfp mouse stained with Ki67 (red) to label proliferative cells.
(B ) Representative femoral bone marrow section from a tamoxifen-treated Nes-CreER T2 ;R26-DTA mouse immunostained with collagen IV antibody (red) to reveal blood vessels (B′ ).
(K ) Representative confocal image of a bone marrow section from a 1-week old (P7) Nes-Gfp Nes-CreER T2 ;R26-Tomato mouse treated with tamoxifeNes-Gfp Nes-CreER
(A-A ′′′) Bone marrow section from newborn Wnt1-Cre 2; R26-Tomato pup stained with calcein to mark calcium deposition (green), showing Wnt1-Cre2 -Tomato+Tosteoblastsoblasts (red) in calcifying areas (asterisks).
Therefore, we harvested femurs from irradiated and transplanted animals at days 7 and 10 post-irradiation and examined bone marrow sections to assess the degree of endothelial repair/regeneration. Figure 6A and B represent low and high magnification images of bone marrow at day 7 and 10 post-irradiation showing gross histological differences between wt and Cxcr2−/− littermates.
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