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Non-amplified RNA from the transduced murine primary bone marrow samples was used for validation with quantitative RT-PCR (qRT-PCR).
In children, microscopic detection on bone marrow samples was positive in 15/18 (83,3%).
The use of marrow samples was approved by The Methodist Hospital Institutional Review Board.
Among the tools used for VL diagnosis, Giemsa staining and microscopic examination of bone marrow samples was the most frequently used technique (48/65 episodes, 73%).
DNA from placenta and bone marrow samples was extracted by using the DNeasy Blood and Tissue Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions.
The chemosensitivity of blood and bone marrow samples was significantly associated (P less than 0.001) in 12 cases where both were tested simultaneously.
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The marrow samples were diluted with DMEM (LG: low glucose) containing 10% FBS.
Bone marrow samples were obtained from nine human donors after informed consent.
Bone marrow samples were tested for the presence of FeLV proviral DNA to determine FeLV latent infection.
In brief, bone marrow samples were accessed from the femurs and tibias of rats and diluted using low glucose DMEM containing 10% FBS.
Patients and methods: Blood samples were collected before and 24 h after cardiac surgery with CPB and bone marrow samples were harvested directly after sternotomy, before initiation of CPB, and at the end of CPB, before sternal closure.
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