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VENTX2 antisera detected a 28-kDa protein in cells transfected with a VENTX2 expression construct, in a human erythroleukemic cell line and in bone marrow samples obtained from patients in recovery phase after chemotherapy.
Pirh2 mRNA expression was also determined in bone marrow samples obtained from patients with MM.
Twenty-six bone marrow samples, obtained from consenting subjects (2 ml each), were also tested.
Bone marrow samples obtained at autopsy were histochemically stained with hematoxylin-eosin and prussian blue reaction.
Bone marrow mononuclear cells (BMMC) were isolated from RA bone marrow samples obtained during joint replacement surgery.
CD34+ cells were purified from bone marrow samples obtained from 49 RA patients and 31 osteoarthritis (OA) patients during joint operations via aspiration from the iliac crest.
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Among the group of 84 patients with a bone marrow sample obtained after chemotherapy, the number of relapses was 11 (25.6%) out of 43 and 6 (14.6%) out of 41 patients (P=0.162) in the DTC and DTC groups, respectively.
Analysis of a bone marrow sample obtained by aspiration indicated an abnormally high proportion of eosinophils (total eosinophils 14%, metamyelocyte eosinophils, 2%; rod nuclear eosinophils, 2%; and leaf nuclear eosinophils, 10%).
It is noteworthy that for one of those two patients, UPN37, the bone marrow sample obtained at diagnosis was correctly classified, even when diluted at 50 and 75percentt (blast content, 20 and 10percentt, respectively).
All patients had a bone marrow sample obtained at the time of the initial presentation immunophenotyped with a "myeloma panel", consisting of antibodies against CD4, CD5, CD8, CD10, CD11b, CD14, CD16, CD19, CD20, CD34, CD38, CD45, CD56, and monoclonal and polyclonal reagents against surface and intracellular kappa and lambda light chains.
We studied gene expression profiles in 30 peripheral blood (PB) and bone marrow (BM) samples obtained from 25 adult AML (acute myeloid leukaemia) patients cured at the Department of Haematology and Bone Marrow Transplantation, Poznan University of Medical Sciences.
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