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Objective: The purpose of this study was to microscopically evaluate a large number of maxillofacial marrow samples for the presence of intramedullary salivary tissue.
We therefore evaluated a specially designed multiplex long-range PCR assay in 65 diagnostic bone marrow samples for its suitability in routine use.
Then the investigators looked at peripheral blood and bone marrow samples for evidence of micronuclei (MN).
No matter CEBPAsingle-mut or CEBPAdouble-mut, the mutation disappeared at complete remission in all patients who had paired bone marrow samples for analysis and reappeared at relapse.
Panhandle PCR method (Felix and Jones, 1998) was also used to analyse the same serial blood and bone marrow samples for MLL rearrangement.
Indeed, Schoenfeld et al (1997) have reported that the concordance between blood and bone marrow samples for occult tumour cell detection was only 6% by immunocytochemistry and 27% by RT-PCR.
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Eleven patients with a haematological malignancy who underwent bone marrow sampling for diagnostic purposes were entered as control patients for cytokeratin-19 (CK-19) relative gene expression (RGE).
In keeping with institutional, federal, and Helsinki Declaration guidelines, all identifiable patients gave written informed consent for undergoing bone marrow sampling for gene expression profiling and the institutional review board of the University of Arkansas for Medical Sciences approved the research studies.
We stained bone marrow samples from both patients for a proliferation marker, Ki-67, and an apoptotic marker.
As we had no permission to take fresh bone marrow samples from neuroblastoma patients for research purposes, we had to use the above-described artificially created "stage 4 bone marrow samples" to test the applicability of polySia-based detection of neuroblastoma cells in fresh samples.
Treatment of TM-233 (2.5 μM for 24 h) was also effective for bone marrow samples from two myeloma patients (Fig. 1e), but TM-233 had no effect on normal human PBMC even in higher doses (up to 10 μM) and with longer exposure (up to 72 h) (Fig. 1f).
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