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Myeloid colony formation of all types responsive to GM-CSF was significantly reduced in OVA/OVA/OVA bone marrow relative to the nontolerized control groups).
Hydroquinone also increases proliferation of CFU-GM progenitor cells in mice with Nf1 null bone marrow relative to WT, the same cell type associated with benzene-associated leukemia.
CMP (common myeloid progenitors) and CLP populations were also present but there was an increased frequency of CMPs in PDK1fl/fl/Vav-Cre+ve bone marrow relative to wild type.
Second, the higher CTC/DTC detection rate may also result from accessibility to large amounts of peripheral blood and bone marrow, relative to the small size of the mouse body.
Importantly, B-ALL cells did not accumulate in vivo and disease did not rapidly progress after Glut1 deletion and numbers of Glut1-deficient B-ALL cells were significantly reduced in spleen and bone marrow, relative to vehicle-treated or control B-ALL.
Similar(55)
To examine the numbers of osteoclast precursors in the marrow, we analyzed the number of granulocyte/macrophage colony forming units (CFU-GMs) and found that there were significantly more colonies per well in the TIEG1−/− marrow cultures relative to WT controls (Figure 1G).
On the other hand, epirubicin treatment caused significant bone marrow suppression relative to the control group in terms of pancytopenia.
In contrast, one study by Kitaeva et al. (1990, abstract only) of Wistar rats exposed to very low concentrations of formaldehyde (0.4 to 1.2 ppm) observed an increased incidence of CA in bone marrow cells relative to controls.
To reduce the associated toxicity, several studies have suggested that weekly doses of docetaxel may help reduce bone marrow suppression relative to 3-week regimens, which show similar efficacy [ 19- 21].
We attribute this to the differences in the CTC/DTC detection methods as well as evaluation of larger blood and bone marrow volume relative to the body size of PDX mice than that of patients, as elaborated here.
In addition, cytogenetic assays in bone marrow of Sprague-Dawley rats (Dallas et al., 1992) exposed to 15 ppm formaldehyde, and mice exposed to formaldehyde via intraperitoneal injection (Natarajan et al., 1983 as cited in US EPA, 2010; Gocke et al., 1981), observed no significant increase in CA or MN in bone marrow cells relative to controls.
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