In the model generated by Mullally et al., quantitative evaluation of bone marrow stem and progenitor populations showed an increase in myeloid progenitors (Lin−Sca1−cKit+), which was largely attributed to an expansion of the megakaryocytic and erythroid progenitor (MEP) subpopulation.
The first is the bone marrow-derived progenitor population of which both hematopoietic stem cells [6] and MSC [12], [21], [22] are suggested as precursors; the second is a tissue-resident MSC population.
CD133 (prominin-1), a 5-transmembrane glycoprotein, was first recognized in CD34+ progenitor populations from adult blood, bone marrow, and fetal liver cells [10].
Osteoclasts that derive from bone marrow progenitor cells via the monocyte lineage are the cell population responsible for erosive changes [ 19, 20] and are pivotally dependent on the triggering of receptor activator of NFκB.
Analysis of blood progenitor populations also showed reduced numbers of MEPs in the bone marrow of knockout animals.
Mice with expression of both heterozygous and homozygous JAK2V617F showed significantly expanded haematopoietic stem and progenitor cell compartments in bone marrow and spleen, as determined by increased frequency of Lin−Sca1+cKit+ (LSK) and myeloid progenitor populations.
Co-culture of hematopoietic progenitors on the mouse bone marrow stromal cell line, OP9, is known to support lymphocyte differentiation from a number of human hematopoietic progenitor populations [29].
Murine bone marrow progenitor cells were cultured in GM-CSF and IL-4 for 7 days to provide a population of DCs.
The contribution of bone marrow-derived cell populations to adult tissue repair has been widely studied, and although controversial, evidence exists implicating various progenitor populations in both tissue remodelling, pathogenic fibrosis, and productive repair.
Briefly, bone marrow progenitor cells were flushed out of the bone marrow using ice-cold RPMI medium.
