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In 30 40% of these patients, individual carcinoma cells can already be detected at the time of primary therapy in cytological bone marrow preparations using immunocytochemistry.
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Animals were sacrificed by CO2 gas inhalation at ~24 h after the final administration, and bone marrow preparations were made using the method of Schmid [ 8].
Other preparations use calcium carbonate.
Large scale preparations used the same conditions.
Histology and skeletal preparations used standard protocols.
Bowel preparations used were polyethylene glycol based.
The procedure of bone marrow aspiration and preparation used in our laboratory has been described previously [ 23].
No tax preparation using Intuit products.
Bone marrow preparations from a wild type mouse were used as a negative control and normal human male preparations were used as a positive control.
Bone marrow-mesenchymal progenitor cells (bm-MPCs; isolated from fresh bone marrow preparations; Lonza Inc., Walkersville, MD, USA) were used as controls.
This population is present in all bone marrow preparations of mice receiving biotin, and can therefore be used as an internal standard to determine the biotin label dilution in individual mice.
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