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Human MSC were isolated from the bone marrow of normal individuals undergoing bone marrow harvest for allogeneic bone marrow transplantation following informed consent, according to institutional guidelines under the approved protocol, as described previously [8].
Viable MSCs were significantly more abundant in the bone marrow of normal than in diabetic mice (31±3 CFU/million nucleated cells vs. 16±2 CFU/million nucleated cells, respectively) (Figure 5A).
Cells from the spleen and bone marrow of normal Rag2-/ mice were used as control.
The percentage of CD138+ cells isolated from bone marrow of normal donors was 0.5∼2% in mononuclear cells.
Further experiments used radiation to wipe out the bone marrow of normal mice, who then received bone marrow transplants from mice that lacked Nur77.
Therefore, we determined the expression of VEGFR-1 and VEGFR-2 in erythroid precursor cells derived from bone marrow of normal mice (Supporting Information Fig. 2), isolated on the basis of CD44 expression and cell size (forward scatter) 34.
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In contrast, there appears to be little or no X chromosome aneuploidy in bone marrow cells of normal females or of females with a haematological malignancy (Secker Walker, 1971) although Y chromosome loss is very frequent in bone marrow of both normal males and those with a haematological malignancy.
Thus, we next analyzed the expression of Ikaros, Aiolos, c-Myc and IRF4 using analytically validated semi-quantitative immunohistochemical assays in primary bone marrow samples of normal (n=10) versus malignant (n=24) plasma cells by co-staining for CD138 and either Ikaros, Aiolos, c-Myc or IRF4, respectively.
The bone marrow of a normal adult produces about 100 billion neutrophils daily.
The present study was designed to assess the numbers/production of NK cells in the spleen and bone marrow of aging, normal mice, after in vivo dietary administration of E. purpurea (14 days), or, after injection of thyroxin, a stimulant of NK cell function (10 days).
To investigate the expression profile of miRNA in pediatric AML and ALL, we used a miRNA microarray to analyze the miRNome expression in 36 newly diagnosed acute leukemia samples and compared them with the mononuclear cells (MNC) from the bone marrow of seven normal donors (Figure 1A and 1B).
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