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No signal was found in blood, muscle, and bone marrow of both groups of mice (Figure 2b).
As shown in Fig 3, compared to age-matched, normal B6 mice, megakaryocytosis and reticulin fibrosis is readily identifiable in the bone marrow of both SH and SPL JAK2V617F- transplanted mice.
Progressive JAK2V617F-driven dinease in Balb/c mice, as exemplified by new bone formation (osteosclerosis) and extensive, nonlinear reticulin fibrosis is similarly seen in the bone marrow of both SH and SPL mice (Fig 3, right panels).
Consistent engraftment of leukemia cells in the bone marrow of both hind limbs was evident even in mice injected with the lowest EM-2eGFPluc dose of 1×105 cells.
As shown in Figure 1C, early pre-plasma cells (CD21−/+CD138int) were found in the bone marrow of both B6 and NZB dTg mice, but NZB dTg mice had a significantly higher proportion of these cells.
Bone marrow of both infected and naïve mice contained large numbers of IBA-1-positive cells.
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Research examining the bone marrow biology of both animals and patients with diabetes confirms that bone marrow function is impaired [ 29] which may reduce cEPCs mobilisation [ 25].
This is perhaps evidence of the bone marrow origin of both populations.
In addition, 122 healthy individuals (volunteer blood/bone marrow donors) of both sexes served as controls.
Finally, compared with (R -[C]PK11195, bone/bone maR -[C]PK11195f bone/bonetracers was significantly lower, providing enhanced contrast.
Marrow cavities of both femur and tibia were flushed with growth medium containing α-MEM (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS, 1% penicillin and streptomycin.
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