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In the first such trial, patients had their own immune cells reduced, with a combination of radiation, chemotherapy, and other treatments, before receiving a donated kidney along with bone marrow from the same donor.
We simultaneously harvested bone marrow from the same donor rat and carried out primary culture of BMSCs (Supplementary Figure 1(a)).
We tried to collect 2 consecutive 10-mL aspirates of bone marrow from the same site, but noted that sometimes only a small volume could be aspirated.
At 6 weeks, engraftment was determined (by flow cytometry) and compared with results in mice injected with marrow from the same patient, but in combination with unmodified HS27a or unmodified HS5 cells.
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Researchers have long known that a follow-up infusion of bone marrow cells from the same donor eases the transition, but they haven't known why.
When the bone marrow cells from the same mouse was stained for human CD45 we observed more human CD45+ cells than HLA+ cells (20% versus 15%) (Figure 3Bi) indicating that some of the human CD45+ cells must be HLA negative.
Bone marrow samples from the same patients (n = 733) had previously been analysed for the presence of DTCs [ 1].
In six cases (31.6%), malignant cells were identified in both bone metastasis and bone marrow samples from the same patient.
Very few hematopoietic cells were detected in mice coinjected with HS5 stroma and marrow cells from the same donors as mice that were coinjected with HS27a cells.
The inclusion of blood and bone marrow cells from the same patients (WM1 and MZL2) was aimed to foresee the extent to which cell origin/developmental stage might have any influence in proteomic profile in a same individual.
We examined two prior bone marrow samples from the same patient (collected at initial diagnosis and 4 months after initial diagnosis, both showing RAEB-1) using Sanger sequencing for the presence of the 14 mutations found in the secondary AML.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com