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Animals were sedated with an intravenous dose (8 mg/kg body weight) of sodium thiopental (Thionembutal®; Abbott Laboratories, São Paulo, Brazil), and bone marrow fluid was removed from the ventral region of the sternum or from the iliac crest.
The medium containing the bone marrow fluid was collected into the syringe, and the process was repeated twice.
The PBS was pushed gently from the syringe into the bone marrow cavity to flush out the bone marrow fluid.
The TB needle is removed, and, with a syringe attached to a needle, a small amount of marrow fluid is removed from the bone marrow.
In bone marrow fluid, VEGF levels increase from 1.5 to 4 p M in acute leukaemia patients (Ye et al, 2003).
Recent studies have revealed that induced marrow fluid pressure and bone strain by mechanical stimulation were dependent on dynamic loading parameters and optimized at certain loading frequencies [ 33].
Similar(51)
Because of the variable manifestations of human brucellosis, a wide array of different clinical specimens may contain viable brucellae, including pus, blood, bone marrow, synovial fluid and tissues, and more rarely, cerebrospinal fluid, urine, and genital exudates.
In particular mesenchymal stem or stromal cells (MSC) identified in a wide range of tissues (including adipose tissue, bone marrow, amniotic fluid, umbilical cord, placenta, menstrual blood and dental pulp) have generated a great deal of clinical interest.
The bacteriophage sensitive strains used during the production and quality control of BFC-1 are P. aeruginosa strain '573', were isolated at the Eliava Institute of Bacteriophage, Microbiology and Virology (EIBMV) in the 1970s from bone marrow interstitial fluid, and S. aureus strain '13 S44 S', isolated at the Brussels Burn Centre in 2006 from a burn wound.
All samples were stored at 4°C. *For all parts, tissue, bone marrow, and fluid from thawing were tested.
MSCs can be isolated from nervous tissue, adipose tissue, bone marrow, amniotic fluid, umbilical cord, placenta, menstrual blood and even dental pulps.
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