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Further restrictions exist for the origin of bovine products (e.g. adeps bovi, brain extract, marrow extract, or neural extract).
Natural substances from animals are, for example, amniotic fluid, aorta extract, Bombyx lipida, brain extract¸ bubulum oil (oil from the feet of cattle), equus extract, heart extract, liver extract, marrow extract, maromota oil, or serum.
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No longer is it necessary to be anesthetized and have bone marrow extracted from your hips.
Murine bone marrow derived macrophages were generated from bone marrow extracted from the femurs of DR3−/− and WT mice, as previously described (21).
Dendritic cells were cultured from tibial and femoral bone marrow extracts of 8 12 week old mice as described [39].
A similar effect was observed using whole bone marrow extracts, isolated thymocytes and purified mature CD3+ splenic T cells suggesting that the altered migration response to CXCL12 is an intrinsic property of the PECAM-1−/− HSCs, and that the defect persists throughout haematopoietic development in lymphocytes.
Briefly, the bone marrow extracts were cultured in non TC-treated polystyrene culture dishes (Corning™) using IMDM (Lonza™) substituted with 10% fetal calf serum (FCS; Hyclone Inc ., ∼1,000 units/ml (20 ng/ml) recombinant mouse granulocyte/macrophage colony stimulating factor (GM-CSF; Pharmingen™), 100 units/ml penicillin, and 100 µg/ml streptomycin.
It also decreased SDF-1 protein levels in bone marrow extracts.
However, multiple attempts to amplify the chimeric MacF1-IRES-tTA transcript from bone marrow extracts from carrier mice by RT-PCR were unsuccessful in identifying this spliced transcript.
TNF treatment of wild-type mice decreased the SDF-1 concentration in bone marrow extracts and decreased the SDF-1 immunostaining of bone marrow stromal cells, and it also increased the circulating OCP numbers.
Repeated attempts to identify these other linked chromosome-4 insertions by our cloning techniques and 5'RACE for trapped sequences in bone marrow extracts were unsuccessful in identifying the origin of this tissue-specific gene-trap tTA expression.
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