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However, tooth has much lower fat content and no marrow compared to bone, which make it easier to be changed into graft material [7].
One could suspect that localization in bone would give a high contribution to bone marrow toxicity, but clinical studies of 223Ra have shown that it is well tolerated by breast and prostate cancer patients [8], with data from repeated dosing suggesting no more damage on red bone marrow compared to placebo [9].
While both C2C12 and ST2 cells are derived from bone marrow, compared to committed bipotential C2C12 cells, ST2 cells are pluripotent mesenchymal stem/stromal cells.
OVA/OVA treatment of WT and STAT6-deficient mice resulted in increased numbers of eosinophil in bone marrow compared to the respective SAL/OVA controls (Figure 2B).
There was no significant difference in size (Figure 5A) or frequency (Figure 5B) of CFU-GM and high proliferative potential colony-forming cells from MacBlue/UAS-Slfn4-V5 bone marrow compared to MacBlue bone marrow.
Finally, when the Chk1+/− bone marrow cells were plated in Methocult supporting the growth of erythroid colonies we saw a decrease in the number of BFU-E colonies from Chk1+/− marrow compared to WT (Figure 1D).
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Yellow marrow appears hypo-intense on b-value images with low ADC values, related to low cell density, with an abundance of fat cells, and reduced water proton diffusivity, due to the hydrophobic nature of fat and lower bone marrow perfusion compared to red marrow.
This might lead to a shift in CD3+ and non CD3+ lymphocytes after allogeneic bone marrow transplantation compared to syngeneic bone marrow transplantation.
Data are illustrated in Figure 1 and Figure 2. A higher number of cells in S-phase were detected in initial ALL bone marrow samples compared to initial AML bone marrow samples (median value of 6.9% in ALL and 5.3% in AML; P<0.05).
FABP4 (fatty acid binding protein 4, adipocyte), perilipin (Plin1), ADFP (Plin2), leptin, adiponectin and C/EBPβ (CCAAT/enhancer binding protein beta) were all much more highly expressed in bone marrow adipocytes compared to the non-floating bone marrow stromal cells, as would be expected for adipocyte specific genes.
Notably, mice treated with zoledronic acid showed significantly lower leukemia burden in bone marrow, spleen and blood, as well as higher numbers of normal bone marrow cells compared to control mice at Day 15 (Fig. 4g, h).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com