To directly test whether the protective effects of PI3Kγ are due to its function in the immune system, we generated bone marrow chimeras.
Spangrude, G. J. Assessment of lymphocyte development in radiation bone marrow chimeras.
J.A. performed experiments and contributed to generating mouse lines and bone marrow chimeras.
Bone marrow chimeras suggested the important of DOCK4 in intrinsic rather than hematopoietic cells in this phenotype.
Taniguchi, H., Abe, M., Shirai, T., Fukao, K. & Nakauchi, H., Ratio is critical for alloreactive T cell deletion and skin graft survival in mixed bone marrow chimeras.
Dexter ,T. M., Moore, M. A. & Sheridan, A. P. Maintenance of hemopoietic stem cells and production of differentiated progeny in allogeneic and semiallogeneic bone marrow chimeras in vitro.
Mice bearing this knockin express DTR in cDCs but not other immune cell populations, and DT injection into zDC-DTR bone marrow chimeras results in cDC depletion.
In bone marrow chimeras, radioresistant and, likely, nonhematopoietic cells were the main source of IFN, but mda5 was required in both marrow-derived and radioresistant host cells for adaptive responses.
Through the generation of a novel knockout animal model and bone marrow chimeras, we assessed the role of this GEF in gut permeability and restoration of barrier function in response to chemical injury to the intestinal epithelium.
Despite evidence of protease activation in the bone marrow, HSPC mobilization by G-CSF or the chemokine Grobeta was abrogated in CXCR4 bone marrow chimeras.
