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These mixed bone marrow chimeras expressed similar numbers of Igha (49.9 ± 6.7)- and Ighb (52.3 ± 7.7 -positive B cells as determined by FACS analysis (data not shown).
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Here, the effects of constitutively active CD40 in DCs on atherosclerosis were examined using low-density lipoprotein-deficient (Ldlr−/−) bone marrow chimeras that express a transgene containing an engineered latent membrane protein 1 (LMP)/CD40 fusion protein conferring constitutive CD40 signaling under control of the DC-specific CD11c promoter (DC-LMP1/CD40).
We cross-transplanted bone marrow in wild-type and NOS3−/− mice with wild-type mice, producing chimeras expressing NOS3 only in vascular endothelium (BC−/EC+) or in both blood cells and vascular endothelium (BC+/EC+).
Reconstitution of bone marrow chimera was verified by flow cytometry analysis: the intrahepatic CD11b+CD45+ cells isolated from Balb/c→B6 chimeras express high levels (>95%) of H2-Kd, and only a small fraction (∼4%) express H2-Kb at levels that are slightly above background.
Different from the hyperglycosylated HSA/GM-CSF chimera expressed in wild-type P. pastoris, the chimera expressed in the och1 deletion strain contained smaller N-glycan.
An A1_14 chimera expressed at similar levels to A1. Chimeras A1_19 and A1_24 showed improved but lower expression than A1_14.
(Activation of this chimera, expressed in CHO cells, was monitored by tyrosine phosphorylation of STAT1).
In order to further investigate the role of PrPC in the LRS we generated bone marrow chimeras with a PrPC-expressing and PrPC-deficient LRS.
The present study was designed to assess the distribution of microglia/macrophages in cerebral ischemia using bone marrow chimera mice known to express enhanced green fluorescent protein (EGFP).
The 4∶1 ratio was needed to ensure that the majority of the non-T cells would be competent in expressing 4-1BB in the 4-1BB−/− TCRα−/− mixed bone marrow chimeras.
More recently, Ribot et al have shown that endogenous Sags expressed by thymic epithelium efficiently select Sag-specific foxp3+ T cells in irradiated bone marrow chimeras [40].
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