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Total bone marrow cellularity was also determined at day 14.

Bone marrow cellularity was normal with brown pigment (likely hemosiderin) and an absence of hemophagocytosis.

The marrow cellularity was assessed under ×100 and ×400 magnifications and described as: aplastic, very low, low, average, high or increased.

Although the overall bone marrow cellularity was not significantly different, we confirmed a significant reduction in recirculating mature B cells in the TACI-Ig mice (Supplementary Table 1).

Differential counts were recorded after a 500-cell count, and marrow cellularity was determined by both BM aspiration and biopsy section.

Further analysis also showed that the number of bone marrow cells that could be obtained from Dnmt2−/− femora was reduced by 20%, indicating that the bone marrow cellularity was significantly (P < 0.05, t-test) reduced in Dnmt2−/− mice (Fig 1D).

Maturation index ratios relating to bone marrow cellularity were calculated with the aim of evaluating bone marrow response in dogs naturally infected with L. chagasi and exhibiting different clinical statuses and patterns of bone marrow parasite density (Table 2).

Bone marrow contains hematopoietic stem cells and stromal cells (mostly adipocytes) [ 8], and marrow cellularity is the volume ratio of hematopoiesis and fat.

A reduction in marrow cellularity is caused by normal aging, but also by a high radiation dose (>12.5 Gy) from Ca, a bone-seeking beta-ray emitter [ 124].

Although no changes in bone marrow cellularity were observed in mice treated with HQ, cellularity was decreased by a factor of two in the mice that had received HQ plus PHE.

Microvessel density, fibrosis, bone growth and marrow cellularity were normalised after plitidepsin treatment of the mice and extramedullary haematopoiesis did not develop in liver; notwithstanding, the abnormally reduced CXCR4 expression in Gata-1 low) Gata-1 low cells was not improgenitorplitidepsin.