Sentence examples for marrow cells using from inspiring English sources
Mononuclear cells were isolated from bone marrow cells using Lymphocyte Separation Medium (Mediatech, Manassas, VA, USA).
Lineage negative (Lin−) cells were enriched from bone marrow cells using the Lineage Cell Depletion kit (Miltenyi Biotec, Auburn, CA) and stained with anti-Sca-1 and c-kit antibodies (BD Biosciences, San Jose, CA).
To define early events in the pathogenesis of this murine model of SA, we compared erythroid differentiation of Sod2-/ and normal bone marrow cells using flow cytometry and gene expression profiling of erythroblasts.
Monocytes/macrophages were isolated from bone marrow cells using a monocyte enrichment kit (StemCell Technologies).
Total RNA was isolated from EML, EPRO and isolated bone marrow cells using trizol reagent (Life Technologies, Gaithersberg, MD).
Total RNA was extracted from AML patients' bone marrow cells using RNeasy minikit (Qiagen, Chatsworth, CA) according to the manufacturer's instructions.
They include Hare's favored mesenchymal stem cells, which can become bone, muscle, and more; a cocktail of bone marrow cells used in German trials; and bone marrow cells that express a certain marker on their surface called CD34.
As the tumor microenvironment is thought to play a strong role in driving the progression of WM, we also investigated whether the five patient-matched CD19-CD138 bone marrow cells used as controls in this study were defined by any mutations not present in a cohort of more than 1000 controls previously sequenced by Mayo Clinic for genetic studies of other disease states.
c-kit+ cells (1 × 10 cells), selected from murine total bone-marrow cells using CD117 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany), were incubated with the retrovirus and RetroNectin (Takara Bio, Madison, WI, USA) for 24 h in StemPro-34 SFM medium (Invitrogen) containing cytokines (20 ng/mL SCF, 10 ng/mL IL-6 and 10 ng/mL IL-3).
Acute haematological toxicity was assessed by daily blood counts, and an assessment of late marrow toxicity made by assaying early marrow progenitor cells using CFUgm assays.
Total RNA was isolated from purified bone marrow CD34+ cells using the Trizol reagent (Life Technologies, Grand Island, NY, USA) according to the manufacturer's instructions.
