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The early biomarkers (1 d after injury) included enhanced responses in γ-H2AX formation in Lin+ bone marrow cells, numbers of splenocytes, lymphocytes, and neutrophils, and concentrations of IL-1β, IL-6, IL-8, and G-CSF in blood.
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The HCE treatment induced a significant decrease on the bone marrow cells number but did not alter the cell number of the spleen and lymph node.
The HCE treatment did not decrease the number of CFU in the peritoneal cavity fluid (Control: 5.6 ± 0.1, HCE 5: 5.5 ± 0.2, HCE 50: 5.5 ± 0.1 Log10) The HCE treatment induced a significant decrease on the bone marrow cells number and in the platelets count in peripheral blood when compared to the control group.
Total bone marrow cell numbers were the same in bone marrow flushes from WT littermates and PECAM-1−/− mice (Figure 1A).
Total bone marrow cell numbers in the PDK1fl/fl/Vav-Cre+ve mice were comparable to control genotypes.
Treatment with 3 × 0.2 μg/kg 1,25- OH 2D3 (Exp 4) had no significant effect on the numbers of ED1 +ve and TRAP/ED1 +ve bone marrow cell numbers (data not shown).
However, when courses of neutropenia following the second course of methotrexate was directly related to the level of depression of bone marrow cell numbers at the time of the second course.
No significant differences were observed between the two groups in the first 48 hours after irradiation except in bone marrow cell numbers, probably due to differences in the overall treatment time between the two TBI schedules.
Consistently, although Nishida et al. [ 26] found markedly reduced total bone marrow cell numbers in a murine CIA model which is similar to patients with RA [ 27], the proportion of cells from the myeloid lineage was found to be elevated in murine arthritis models [ 6, 28].
This situation leads to increased numbers of OCPs in both bone marrow and blood, whereas SDF-1 or G-CSF administration triggers a rapid release of cells from the bone marrow – and the total bone marrow cell number is consequently reduced.
Although STAT5−/− mice showed a ∼20% reduction in the total number of bone marrow cells (Figure 2B), the absolute number of mature neutrophils (CD11b+Gr-1hi) in the bone marrow of these mice was significantly increased relative to wild-type littermates (Figure 2C).
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