Control mice received bone marrow cells only.
We lethally irradiated BALB/c mice, in which we then transferred myeloprotective cells from T cell depleted (TCD) B6 bone marrow cells (BMCs) alone (BM group) or along with allogeneic T cells from B6 wild-type (WT) (WT T group) or B6 p110δ D910A (D910A T group) mice.
Hao et al.[ 133] performed a similar experiment with bone marrow deficient in myeloid differentiation factor 88, and reported that the deficiency (in bone marrow-derived cells alone) markedly reduced amyloid burden.
In the absence of activated bone marrow cells, recombinant granulin alone was able to stimulate the growth of indolent tumor cells with an accompanying desmoplastic reaction.
Otherwise, non-adherent bone marrow cells were cultured alone with 10 ng/ml of M-CSF (R & D Systems, Minneapolis, MN, USA) for two days, and then adherent cells were cultured with 100 ng/ml of soluble RANKL (sRANKL) (R & D Systems) for three days.
When the bone marrow cells were treated with PTH alone, the non-adherent cell pool was not amplified.
Evidence that at least M-CSF signaling is blocked in the absence of SHP-2 was provided by experiments in which bone marrow cells were treated with this cytokine alone (Fig. 7).
Using this previously validated assay, human bone marrow cells were grown in culture with either spirulina alone, NT-020 alone, or spirulina in combination with NT-020 added to the culture media for 72 hours.
However, bone marrow cells of murine transduced with Evi-1 alone cannot cause leukemia [ 3], while deletion of RUNX1 alone does not immortalize bone marrow cells [ 4], which suggest that both suppression of RUNX1 and activation of Evi-1 are required for RUNX1-Evi-1 leukemogenesis.
Donating bone marrow is perfectly safe, and bone marrow is entirely renewable -- donated marrow cells regenerate within a matter of weeks.
