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The primary objective of this study was to determine the effects of in vivo scanning at 2-week intervals on bone healing of a critical sized radial defect in rabbits and to investigate the effect of this radiation protocol on bone marrow cell viability using clinically applicable radiation doses.
DLB trained and assisted KRS with cytopreps of bone marrow and evaluated smears for marrow cell viability.
Although 17-DMAG did not significantly improve the bone marrow cell viability at day 3 compared to irradiated vehicle-treated animals, 17-DMAG did markedly promote bone marrow cell viability at day 7 (17-DMAG/RI vs. vehicle/RI: 6.36 ± 1.75 × 10 vs. 2.0 × 10 ± 2.48 × 10) and day 15 (8.26 ± 1.84 × 10 vs. 3.46 × 10 ± 2.91 × 10) after irradiation.
After erythrocytes were lysed by red blood cell lysis buffer (BioLegend San Diego, CA, USA), total bone marrow cell viability from each mouse was assayed by trypan blue staining [ 12].
The consistent results from both methods indicate that 17-DMAG protects bone marrow cells from irradiation, suggesting the improved bone marrow cell viability may contribute to increased 30-day survival in irradiated mice pretreated with 17-DMAG.
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We used a monoenergetic (70 KeV) X-ray sources that was reported to be safe for both structural bone endpoints and bone marrow cell viabilities in small animals [91].
Flow cytometry was performed to analyze the bone marrow aspirate and the bone marrow concentrate for cell viability and number of total nucleated cells and hematopoietic CD34+ cells (Stem-Kit; Beckman Coulter, Ref IM3630).
Flow cytometry was performed to analyze the bone marrow aspirate and concentrate for cell viability and number of total nucleated cells and hematopoietic CD34+ cells (Stem-Kit; Beckman Coulter, Ref IM3630).
We also evaluated cell viability in bone marrow stromal cells and MLO-y4 osteocytes treated with dexametasone with or without risedronate.
We studied superparamagnetic iron oxides (SPIO) for labeling human bone marrow-derived mesenchymal stem cells (hBMSCs) regarding effectivity, cell viability, long term metabolic cell activity, chondrogenic differentiation and hBMSC secretion profile.
At the time of cell thaw at each clinical site, samples of the MSCs are taken by the bone marrow transplantation laboratory for standardized quantitation of cell viability and for cell sterility assays.
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marrow cell death
marrow cell transplantation
marrow cell migration
marrow cell therapy
marrow cell differentiation
marrow cell seeding
marrow cell homeostasis
marrow cell growth
marrow cell culture
marrow cell mobilization
marrow cell morphology
marrow cell invasion
marrow cell implantation
marrow cell adhesion
marrow cell pellet
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