Sentence examples for marrow cell suspensions from inspiring English sources

Briefly, bone marrow cell suspensions from P38α+/− and P38α−/− mice were pre-treated with rat anti-mouse CD16/CD32 antibody (2.4G2, BD Biosciences), and subsequently stained with APC-conjugated anti-mouse Ter119 (BD Biosciences,1 50) and FITC-conjugated anti-mouse CD71 (BD Biosciences,1 50) for 30 min in the dark at 4 °C.

While initial procedures were taking advantage of 5-Fluorouracil (5-FU) treatment of RAG-deficient or SCID donor mice as source of haematopoietic stem cells, we used bone marrow cell suspensions enriched in lineage antigen-negative (Lin−) cells from untreated donors for TCR retrogenesis.

Marrow cell suspensions (500 µL; 1×106 cells) were plated in low M-CSF [3% L 929 cell-conditioned medium (L-CM) as a source of M-CSF] in RPMI containing 20% FBS.

RBC-depleted bone marrow cell suspensions were labeled with a biotinylated TER-119 mAb; TER-119+ cells were pulled down with streptavidin MicroBeads (Miltenyi Biotec) according to the manufacturer's instructions.

The higher expression of Tfrc found in Sod2-/ erythroblasts was verified both by FACS analysis of CD71 surface expression on bone marrow cell suspensions (figure 4a) and by qPCR (figure 4b).

Bone marrow cell suspensions were labeled with a purified anti-CD16/32 anti-CD16/32 surface Fc fragmAbt receptors (for) saturation, FITcellnjugated anti-CD71 and PE-conjugated anti-TER-119 mAbsurfacee-cold Fcuorescence-activated cell sorting (fragmentffereceptorsDTA: 0.5% BSA: PBS).

The bone marrow cell suspension was centrifuged at 300 × g for 5 min. The cells were collected, suspended in PBS by addition of red blood cell lysate for depletion of erythrocytes, and incubated at 37.0°C for 8 min away from light.

Strikingly, both markers were significantly elevated in the bone marrow cell suspension of rats with acute arthritis.

The bone marrow cell suspension was then carefully layered over 3 ml of 1.077 g/ml Percoll solution in a 10 ml centrifuge tube.

Bone marrow cell suspension was prepared by flushing the femurs with IMDM 2% foetal bovine serum (FBS) and spleen cell suspension was prepared by mincing the spleen with scissors and aspirating the pieces up and down through a 1cc syringe in IMDM 2% FBS.

However, aspiration for flow cytometry is the sole method in clinical and research use and the dilution of the bone marrow cell suspension by peripheral blood would only slightly decrease the significant differences reported here, and would not lead to false-positive results.