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Bone marrow cell populations were quantified and phenotyped using a CyAn ADP flow cytometer (Dako, Ely, UK).
It was previously described that some bone marrow cell populations, including MSC, express neural markers even before any neural induction protocol [3], [38] [42].
Cells were then immediately stained with PE conjugated Sca-1 and FITC conjugated CD90.2 antigens (BD Bioscience) to detect progenitor marrow cell populations [39], [40].
Failure of the bone structure to recover on reambulation may in part be caused by the collapse of the osteogenic potential of bone marrow cell populations during disuse.
Here, we tested whether specific bone marrow cell populations as well as trabecular bone morphology can benefit from the application of low-level whole body vibrations during disuse and reambulation.
It serves as another step towards a comprehensive model of hematopoiesis comprising models of stem cell regulation, lineage commitment, bone marrow cell populations, growth-factors and chemotherapy actions.
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In this study, the orientation-regulated immobilization of a Notch ligand was designed to achieve the efficient Notch ligand receptor recognition for the ex vivo proliferation of a bone marrow cell population containing HSC.
Proportions of Sca-1 and CD90.2 positive cells to the total viable marrow cell population were reported (n = 6 per group and time point).
Thus, protecting the regenerative potential of the bone marrow cell population during the challenge of disuse may be critical for tissue recovery during reambulation.
After testing six different sorted subpopulations of GFP positive bone marrow on GFP negative animals, we found that the bone marrow cell population Sca+/Mac-1− can migrate into islets more efficiently than WBM and the other five cell subpopulations.
Supporting the hypothesis that the ability of bone to recover will correlate with the status of the bone marrow cell population, subsequent experiments showed that vibrations applied during disuse, rather than reambulation, were more effective in altering bone morphology upon reambulation.
More suggestions(15)
marrow cell counts
marrow cell aspirates
marrow cell precursors
marrow cell numbers
marrow neutrophil populations
marrow cell BMCs
marrow side populations
marrow cell lines
marrow cell metaphases
marrow precursor populations
marrow progenitor populations
marrow cell types
marrow cell donors
marrow cell cultures
marrow cell suspensions
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