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MTT assay was used for bone marrow cell lines (KG-1 and HBMF-SPH cell lines) to evaluate cytotoxicity of CLs and CL/miR-101.
Sung et al. assembled this μCCA system for hydrogel cell culture of liver, tumor, and bone marrow cell lines.
Supernatants of multiple myeloma cells and human myeloma cell lines induce significantly higher in vitro angiogenesis compared with normal bone marrow cell lines.
In bone marrow cell lines, 336 identified miRNAs were expressed in 3,390 isoforms and the number of different isomiRs for a given miRNA ranged from 1 to 74 (Kuchenbauer et al. 2008).
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Our data suggest that PPARγ2 may have adipogenic and anti-osteogenic potential, while PPARγ1 may stimulate osteoblastogenesis, a possibility supported by results from microarray analysis of the mouse bone marrow cell line U-33 ectopically overexpressing PPARγ.
As this study was directed initially toward identification of genes that might influence human B lymphopoiesis, RT-PCR analysis of FIGLER gene expression was performed on fetal bone marrow B lymphocyte subpopulations, bone marrow stromal cell lines, fetal thymus, and the non-B cell fraction in bone marrow.
The human marrow stroma cell lines HS5 and HS27a, derived from the marrow of a healthy volunteer and immortalized by transduction with human papilloma virus E6/E7 constructs, were a gift from Dr Torok-Storb (FHCRC, Seattle, WA, USA).
To show that uPAR-positive cells possess high clonogenic and self-renewal potential, we sorted primary lung (H1417, H69AR), metastatic bone marrow (H211) cell lines by FACS using anti-uPAR-FITC-conjugated monoclonal antibodies.
In bone marrow stromal cell lines, endoglin was shown to stimulate proliferation [ 24].
Stromal support of CLL-BC can be reproduced when they are co-cultured with bone marrow stromal cell lines.
The RAJI human B-cell line, the MS5 murine and HS5 human bone marrow stromal cell lines were purchased from DSMZ cell depository bank.
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