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Several analyses were performed including histological examination, nucleated marrow cell counts, colony forming unit assay (CFUs), and stem cell quantification.
Differential bone marrow cell counts revealed increases in the myeloid:erythroid ratio associated to increased numbers of granulopoietic cells in the different clinical groups compared with non-infected dogs.
Nucleated bone marrow cell counts were also reduced in the WT offspring of P2Y14 dams exposed in utero to IR.
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We did not observe any biological difference between fractured and non-fractured patients in terms of 25 hydroxyvitamin D, PTH, calcemia, β2 microglobulin, monoclonal protein value and marrow cells count.
Total nucleated bone marrow (BM) cell counts as well as spleen and thymus weights are reduced in mutant animals, which maintain normal peripheral blood cell counts.
Characterise the bone marrow haematological changes in severe sepsis/septic shock patients and to evaluate the prognostic value of the marrow cell differential count (myeloid, lymphoreticular, erythroid series).
Compared to a healthy female donor the patient had a 4-fold reduced bone marrow mononuclear cell count (1.53×10/ml vs. 0.4×10/ml) with reduced viability after gradient centrifugation (98.1percentt vs. 84.3percentt viable cells).
Using pain and functional outcome measures, a receiver operating characteristic analysis was used to define an optimal clinical outcome threshold at which bone marrow nucleated cell count could be divided into either a lower or higher cell count group within a treatment protocol.
Fresh bone marrow cells were counted using a hemocytometer, centrifuged for 5 minutes at 200× g at 4°C and gently resuspended to obtain a solution containing from 4 to 6×106 cells/ml (unless otherwise mentioned) in freezing media containing 90% fetal bovine serum and 10% DMSO (unless otherwise mentioned).
On day 7, bone marrow cells were counted with trypan blue exclusion using a hemocytometer to assess survival.
All mice were killed one month after TBI for analysis of peripheral blood cell counts, bone marrow cellularity and long-term HSC (CD34-lineage-sca1+c-kit+) frequency.
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