Hirohata and colleagues [ 23], in a highly accessed article published in Arthritis Research and Therapy in early 2006, described a study of bone marrow cells aspirated from the iliac crests of RA patients.
Briefly, primary bone marrow cells aspirated from the humeral heads of rabbits were cultured in regular medium consisting of Earle's Minimal Essential Medium (Nacalai Tesque, Kyoto, Japan) with 15% fetal bovine serum (JRH Bioscience, Lenexa, KS) and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin; Nacalai Tesque) for 2 weeks [ 18, 19].
Briefly, marrow cells were aspirated using a 25-gauge needle and a 5 mL syringe containing media.
Bone marrow cells were aspirated from the iliac crest of healthy adult donors with their informed consent and the approval of the Regional Ethics Committee (REK # S-90128).
Conclusion We recommend collection volumes of bone marrow aspirates of at least 8 mL to reduce the risk of obtaining aspirates with low cell numbers.
Bone marrow cells were isolated from whole marrow aspirates of the iliac crest of each of three healthy male donors (AllCells, Berkeley, CA) and plated as in Fig. 1A.
Donor marrow cells from DLA-nonidentical donors were aspirated under general anesthesia through needles inserted into humeri and femora and stored in heparinized tissue culture medium at 4°C for no more than 6 hours.
In a similar model, Saw and colleagues [ 69] used bone marrow aspirate cell collections injected weekly with HA.
This study examines the osteogenic potential of PLAs and bone marrow aspirate cells (BMAs), when exposed to either recombinant human bone morphogenetic protein (BMP -2 (rh-BMP -2 or adenovirus containing BMP-2 cDNA (Ad-BMP-2).
To assure absence of contaminating plasma cells, bone marrow aspirates were also stained for the plasma cell marker CD38 using anti-CD38-PE and analyzed by flow cytometry (see Additional file 1).
