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b After 15 days, the marrow cavity was filled with woven bone and trabecular bone (X200).
By histological examination, we observed that spongy bone accumulated in Mmp13−/− calluses while the reconstitution of the bone marrow cavity was more advanced in WT calluses (Fig. 4A, day 28, middle column; day 56, right column).
The anabolic effect of PTH on wt tibiae was so dramatic that the majority of the bone marrow cavity was replaced by newly formed bone (Fig. 2A and B).
Histologically, the marrow cavity was reestablished, with intertrabecular spaces being filled with adipose marrow and with evidence of focal hematopoiesis.
The bone marrow cavity was flushed out under aseptic conditions with alpha IMDM culture medium [ 34, 35].
The bone ends were cut off with scissors and the marrow cavity was flushed slowly by injecting Dulbecco's Modified Eagle's Medium (DMEM; Invitrogen, Carlsbad, CA).
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Corticomedullary margins in the diaphysis are blurred, and the marrow cavity is obliterated.
The marrow cavity is filled with sediment (gray in this image; http://www.neoucom.edu/audience/about/departments/anatomy/AnatFaculty/media, © J.G.M. Thewissen).
Therefore, the mechanical ablation of the rat femoral marrow cavity is an established and suitable model for gene expression profiling studies regarding intramembranous bone regeneration.
The contents of the marrow cavity were disrupted mechanically and reamed by hand with a 2.0 mm drill bit proximally up to the lesser trochanter.
Bone inside the total marrow cavity is considered trabecular bone, the remainder is regarded as cortex.
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